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Importance of P16ink4a marker in oral neoplasms

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Original Article

Author Details : Uma Rani Pallivilla, Gudeli Vahini*, Anjana Priyanka Aluri, Rajendra Prasad Jalagam

Volume : 8, Issue : 1, Year : 2023

Article Page : 10-13

https://doi.org/10.18231/j.achr.2023.003

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Abstract

Context: We have increase in number of oral lesions at ASRAMS, as it is a referral centre and there by we have determined to study the prevalence of these lesions and its association with P16ink4a marker.
Aims and Objectives: The purpose of this study is to evaluate the role of P16ink4a in oral epithelial dysplasias and oral squamous cell carcinoma by immunohistochemistry.
Settings: In clinically significant patients, correlation between histopathology findings and its association with P16ink4a marker has been studied in Department of Pathology, ASRAMS.
Study design: A retrospective study done from July 2017 to July 2019.
The study was carried out after approval of the Institutional Ethics Committee. The biological material received was resection specimen and punch biopsies of the lesion which was fixed in 10% formalin for 6hrs later processed, followed by paraffin embedding and hematoxylin & eosin staining of the sections. Histopathological classification of lesions was done according to the criteria proposed by WHO for oral cavity lesions.
Immunohistochemical analysis with P16ink4a was performed on the serial sections. Development of reactions was performed with DAB (Diamino benzidine). The panel used was clone E6H4, requiring no dilution followed by epitope retrieval solution.
Statistical analysis: All data were tabulated in a Windows Excel spreadsheet (Microsoft Excel 2011) and statistically analyzed using software version. The expression of P16ink4a in various lesions was tabulated.
Results: The study included 29 cases of oral lesions, most of them belonged to age group of 40 to 60 yrs. Male preponderance is noted. Most of the cases in our study were located on tongue. Histopathologically well differentiated squamous cell carcinomas were more in number. The immunoexpression of p16 stain was present at nuclear and cytoplasmic level mainly found at the basal dysplastic epithelium. P16 had a weak intensity at level of tumor proper and also at invasion front.
Conclusion: P16ink4a immunohistochemical marker can be used as a surrogate biomarker for HPV detection of oral epithelial dysplasias and oral squamous cell carcinomas. P16 is useful marker in identifying dysplastic lesions and decrease of its immunoexpression is a predictive factor notified for neoplastic transformation.
 

Keywords: Oral, Squamous, Neoplasm

How to cite : Pallivilla U R, Vahini G, Aluri A P, Jalagam R P, Importance of P16ink4a marker in oral neoplasms. IP Arch Cytol Histopathol Res 2023;8(1):10-13

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